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A simple sperm wash takes about 20 to 40 minutes. After it is spun, active, healthy sperm will make their way to the very bottom layer of liquid in the test tube .

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Description:This sperm is then used in the IUI procedure. Jaypee Brothers Medical Publishers; ; p. As the sperm mixture is spun, sperm cells fall to the bottom of the test tube, producing a mass of dense, highly active sperm. Sperm concentrations below 5x have been associated with low counts of sperm with normal morphology, and impaired sperm motility in such cases is suggestive of lower chances of fertilization Wainer et al. If one chooses to consider only the cost of the materials used during each method, discontinuous density gradient centrifugation is more expensive, as also observed by Neves When used in IUI, these techniques are expected to provide at least five million motile spermatozoa.

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